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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate recovered from a patient with meningitis. The isolate shows reduced susceptibility to penicillin (MIC = 2 µg/mL). The MOST likely mechanism for this reduced susceptibility is:

A) Alterations in the penicillin-binding proteins (PBPs) that reduce their affinity for penicillin.
B) Production of beta-lactamase enzymes that hydrolyze penicillin.
C) Increased activity of efflux pumps that actively remove penicillin from the cell.
D) Modification of the peptidoglycan structure, preventing penicillin binding.

2. A research laboratory is studying the pathogenesis of respiratory syncytial virus (RSV) infection in infants.
They find that a specific viral surface glycoprotein is primarily responsible for attachment to host cells. This glycoprotein is:

A) Neuraminidase (N)
B) Matrix protein (M)
C) Hemagglutinin (H)
D) Fusion protein (F)

3. The erm gene in Staphylococcus aureus confers resistance to which class(es) of antibiotics via modification of the ribosomal target site?

A) Beta-lactams only
B) Fluoroquinolones
C) Aminoglycosides
D) Macrolides, Lincosamides, and Streptogramin B (MLS<sub>B</sub>)

4. A research team is investigating the mechanism of bacterial resistance to a novel fluoroquinolone antibiotic.
They isolate a resistant strain of Escherichia coli from a patient with a urinary tract infection. Genetic analysis of this strain reveals a point mutation in the gyrA gene. This mutation MOST likely results in:

A) Production of an enzyme that inactivates the fluoroquinolone molecule.
B) Alteration of the DNA gyrase enzyme, reducing its affinity for the fluoroquinolone.
C) Modification of the ribosomal target site, preventing antibiotic binding.
D) Increased expression of efflux pumps that actively transport the antibiotic out of the cell.

5. A clinical microbiology laboratory is monitoring the emergence of resistance to colistin in Gram-negative bacteria. Resistance to colistin is often mediated by modifications of the bacterial lipopolysaccharide (LPS).
The genetic basis for acquired colistin resistance most commonly involves:

A) Acquisition of genes encoding efflux pumps that specifically target colistin.
B) Production of enzymes that directly inactivate the colistin molecule.
C) Mutations in genes encoding enzymes that modify the lipid A component of LPS.
D) Alterations in the outer membrane porin proteins, reducing colistin uptake.

Solutions: